Functional Analysis of the Channelrhodopsin Genes from the Green Algae of the White Sea Basin

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Olga V. Karpova^1,a*, Elizaveta N. Vinogradova^1,2, Anastasiya M. Moisenovich¹, Oksana B. Pustovit¹, Alla A. Ramonova¹, Denis V. Abramochkin¹, Elena S. Lobakova¹

¹Division of Biology, Lomonosov Moscow State University, 119991 Moscow, Russia

²Genome Center, National Research Center “Kurchatov Institute”, 123182 Moscow, Russia

^* To whom correspondence should be addressed.

Received: March 3, 2024; Revised: May 31, 2024; Accepted: June 19, 2024

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Optogenetics, the method of light-controlled regulation of cellular processes is based on the use of the channelrhodopsins that directly generate photoinduced currents. Most of the channelrhodopsin genes have been identified in the green microalgae Chlorophyta, and the demand for increasing the number of functionally characterized channelrhodopsins and the diversity of their photochemical parameters keeps growing. We performed the expression analysis of cation channelrhodopsin (CCR) genes in natural isolates of microalgae of the genera Haematococcus and Bracteacoccus from the unique Arctic Circle region. The identified full-length CCR transcript of H. lacustris is the product of alternative splicing and encodes the Hl98CCR2 protein with no photochemical activity. The 5′-partial fragment of the B. aggregatus CCR transcript encodes the Ba34CCR protein containing a conserved TM1-TM7 membrane domain and a short cytosolic fragment. Upon heterologous expression of the TM1-TM7 fragment in CHO-K1 cell culture, light-dependent current generation was observed with the parameters corresponding to those of the CCR. The first discovered functional channelrhodopsin of Bracteacoccus has no close CCR homologues and may be of interest as a candidate for optogenetics.

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KEY WORDS: channelrhodopsins, green algae, photo-induced current, optogenetics

DOI: 10.1134/S0006297924080030

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