REVIEW: Catalytic and Electrocatalytic Mechanisms of Cytochromes P450 in the Development of Biosensors and Bioreactors

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Polina I. Koroleva¹, Tatiana V. Bulko¹, Lyubov’ E. Agafonova¹, and Victoria V. Shumyantseva^1,2,a*

¹Institute of Biomedical Chemistry, 119121 Moscow, Russia

²Pirogov Russian National Research Medical University, 117997 Moscow, Russia

^* To whom correspondence should be addressed.

Received June 22, 2023; Revised August 23, 2023; Accepted August 25, 2023

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Cytochromes P450 are a unique family of enzymes found in all Kingdoms of living organisms (animals, bacteria, plants, fungi, and archaea), whose main function is biotransformation of exogenous and endogenous compounds. The review discusses approaches to enhancing the efficiency of electrocatalysis by cytochromes P450 for their use in biotechnology and design of biosensors and describes main methods in the development of reconstituted and electrochemical catalytic systems based on the biochemical mechanism of cytochromes P450, as well as and modern trends for their practical application.

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KEY WORDS: cytochrome P450, mechanism of catalysis, bioelectrochemistry, electrocatalysis

DOI: 10.1134/S0006297923100176

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