Formation of New Polysomes on Free mRNAs in a Cell-Free Translation Systems Is Accompanied by Partial Disassembly of Previously Formed Polysomes

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E. A. Sogorin, S. Ch. Agalarov, and A. S. Spirin^*

Institute of Protein Research, Russian Academy of Sciences, 142290 Pushchino, Moscow Region, Russia; fax: +7 (495) 514-0218; E-mail: spirin@vega.protres.ru

^* To whom correspondence should be addressed.

Received May 6, 2015

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A method for detection of the fluorescence-labeled mRNA in translating ribosomal complexes has been developed. It is demonstrated that in the working cell-free translation system with preformed polysomes, formation of new polysomes on free mRNA takes place. For the first time, it is shown that the process is accompanied by partial disassembly of the previously formed polysomes. This result is interpreted as an indication of the direct relationship between processes of translation termination of polysomal ribosomes and translation initiation of free mRNAs.

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KEY WORDS: eukaryotic polyribosomes, mRNA, cell-free translation system, fluorescence

DOI: 10.1134/S0006297915100144

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