Biogenesis of Chlorophyll-Binding Proteins under Iron Stress in Synechocystis sp. PCC 6803

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X. Liu, J. Zhao, and Q. Wu^*

Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, P. R. China; fax: 86-10-62781825; E-mail: qingyu@tsinghua.edu.cn

^* To whom correspondence should be addressed.

Received June 3, 2005; Revision received June 27, 2005

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The biogenesis of chlorophyll-binding proteins under iron stress has been investigated in vivo in a chlN deletion mutant of Synechocystis sp. PCC 6803. The chlN gene encodes one subunit of the light-independent protochlorophyllide reductase. The mutant is unable to synthesis chlorophyll in darkness, causing chlorophyll biosynthesis to become light dependent. When the mutant was propagated in darkness, essentially no chlorophyll and photosystems were detected. Upon return of the chlN deletion mutant to light, 77 K fluorescence emission spectra and oxygen evolution of greening cells under iron-sufficient or -deficient conditions were measured. The gradual blue shift of the photosystem I (PS I) peak upon greening under iron stress suggested the structural alteration of newly synthesized PS I. Furthermore, the rate of biogenesis of PS II was delayed under iron stress, which might be due to the presence of IsiA.

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KEY WORDS: chlorophyll synthesis, chlorophyll-binding proteins, cyanobacterium, fluorescence emission, iron stress, IsiA

DOI: 10.1134/S0006297906130177

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