REVIEW: Methods for Assessment of Nucleotide Excision Repair Efficiency

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Aleksei A. Popov¹, Irina O. Petruseva¹, Natalya V. Naumenko¹, and Olga I. Lavrik^1,2,a*

¹Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, 630090 Novosibirsk, Russia

²Novosibirsk National Research State University, 630090 Novosibirsk, Russia

^* To whom correspondence should be addressed.

Received March 29, 2023; Revised August 30, 2023; Accepted August 31, 2023

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Nucleotide excision repair (NER) is responsible for removing a wide variety of bulky adducts from DNA, thus contributing to the maintenance of genome stability. The efficiency with which proteins of the NER system recognize and remove bulky adducts depends on many factors and is of great clinical and diagnostic significance. The review examines current concepts of the NER system molecular basis in eukaryotic cells and analyzes methods for the assessment of the NER-mediated DNA repair efficiency both in vitro and ex vivo.

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KEY WORDS: nucleotide excision repair, DNA damage, methods for determining NER activity

DOI: 10.1134/S0006297923110147

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