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REVIEW: Modern Methods for Assessment of microRNAs


Oleg L. Bodulev1 and Ivan Yu. Sakharov1,a*

1Lomonosov Moscow State University, Faculty of Chemistry, 119991 Moscow, Russia

* To whom correspondence should be addressed.

Received January 21, 2022; Revised March 21, 2022; Accepted March 21, 2022
The review discusses modern methods for the quantitative and semi-quantitative analysis of miRNAs, which are small non-coding RNAs affecting numerous biological processes such as development, differentiation, metabolism, and immune response. miRNAs are considered as promising biomarkers in the diagnosis of various diseases.
KEY WORDS: bioanalysis, microRNA, nucleic acids, amplification, polymerases

DOI: 10.1134/S0006297922050042