Effect of Trehalose on Oligomeric State and Anti-Aggregation Activity of αB-Crystallin

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Natalia A. Chebotareva^1,a*, Tatiana B. Eronina¹, Valeriya V. Mikhaylova¹, Svetlana G. Roman¹, Kristina V. Tugaeva¹, and Boris I. Kurganov^1#

¹Bach Institute of Biochemistry, Federal Research Centre “Fundamentals of Biotechnology” of the Russian Academy of Sciences, 119071 Moscow, Russia

^* To whom correspondence should be addressed.

^# Deceased.

Received September 20, 2021; Revised October 21, 2021; Accepted October 22, 2021

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αB-Crystallin (αB-Cr), one of the main crystalline lens proteins, along with other crystallins maintains lens transparency suppressing protein aggregation and thus preventing cataractogenesis. αB-Cr belongs to the class of molecular chaperones; being expressed in many tissues it has a dynamic quaternary structure, which is essential for its chaperone-like activity. Shift in the equilibrium between ensembles of oligomers of different size allows regulating the chaperone activity. Trehalose is known to inhibit protein aggregation in vivo and in vitro, and it is widely used in biotechnology. The results of studying the effect of trehalose on the chaperone-like activity of crystallins can serve as a basis for the design of drugs delaying cataractogenesis. We have studied the trehalose effect on the quaternary structure and anti-aggregation activity of αB-Cr using muscle glycogen phosphorylase b (Phb) as a target protein. According to the dynamic light scattering data, trehalose affects the nucleation stage of Phb thermal aggregation at 48°C, and an increase in the αB-Cr adsorption capacity (AC₀) is the main effect of trehalose on the aggregation process in the presence of the protein chaperone (AC₀ increases 1.5-fold in the presence of 66 mM trehalose). According to the sedimentation analysis data, trehalose stabilizes the dimeric form of Phb at the stages of denaturation and dissociation and enhances the interaction of αB-Cr with the target protein. Moreover, trehalose shifts the equilibrium between the αB-Cr oligomers towards the smaller forms. Thus, trehalose affects the quaternary structure of αB-Cr and increases its anti-aggregation activity at the nucleation stage.

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KEY WORDS: αB-crystallin, anti-aggregation activity, trehalose, glycogen phosphorylase b, dynamic light scattering, analytical ultracentrifugation

DOI: 10.1134/S0006297922020043

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