REVIEW: Nonspecific Synthesis in the Reactions of Isothermal Nucleic Acid Amplification

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Nadezhda V. Zyrina^1,2 and Valeriya N. Antipova^2,a*

¹Institute of Protein Research, Russian Academy of Sciences, 142290 Pushchino, Moscow Region, Russia

²Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, 142290 Pushchino, Moscow Region, Russia

^* To whom correspondence should be addressed.

Received February 2, 2021; Revised April 16, 2021; Accepted April 16, 2021

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The review focuses on the main factors involved in the formation of nonspecific products in isothermal nucleic acid amplification, such as mispriming, ab initio DNA synthesis, and additional activities of DNA polymerases, and discusses approaches to prevent formation of such nonspecific products in LAMP, RPA, NASBA, RCA, SDA, LSDA, NDA, and EXPAR.

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KEY WORDS: isothermal amplification, background synthesis, DNA polymerases, LAMP, SDA, EXPAR

DOI: 10.1134/S0006297921070099

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