Direct Effect of the Synthetic Analogue of Glucagon-Like Peptide Type 1, Liraglutide, on Mature Adipocytes Is Realized through Adenylate-Cyclase-Dependent Enhancing of Insulin Sensitivity

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Elizaveta D. Mamontova^1,2,3, Svetlana S. Michurina^1,2, Iurii S. Stafeev^2,a*, Ekaterina L. Sorkina³, Igor A. Sklyanik³, Ekaterina O. Koksharova³, Mikhail Y. Menshikov², Marina V. Shestakova³, and Yelena V. Parfyonova^2,4

¹Faculty of Biology, Lomonosov Moscow State University, 119234 Moscow, Russia

²Institute of Experimental Cardiology, National Medical Research Centre for Cardiology, 121552 Moscow, Russia

³Diabetes Institute, Endocrinology Research Centre, 117036 Moscow, Russia

⁴Faculty of Basic Medicine, Lomonosov Moscow State University, 119234 Moscow, Russia

^* To whom correspondence should be addressed.

Received November 2, 2020; Revised December 7, 2020; Accepted December 7, 2020

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Incretin hormones analogues, including glucagon-like peptide type 1 (GLP-1), exhibit complex glucose-lowering, anorexigenic, and cardioprotective properties. Mechanisms of action of GLP-1 and its analogues are well known for pancreatic β-cells, hepatocytes, and other tissues. Nevertheless, local effects of GLP-1 and its analogues in adipose tissue remain unclear. In the present work effects of the GLP-1 synthetic analogue, liraglutide, on adipogenesis and insulin sensitivity of the 3T3-L1 adipocytes were examined. Enhancement of insulin sensitivity of mature adipocytes by the GLP-1 synthetic analogue liraglutide mediated by adenylate cyclase was demonstrated. The obtained results imply existence of the positive direct insulin-sensitizing effect of liraglutide on mature adipocytes.

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KEY WORDS: insulin resistance, adipocyte, GLP-1, incretins

DOI: 10.1134/S000629792103010X

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