Iron Metabolism after Application of Modified Magnetite Nanoparticles in Rats

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I. V. Milto^1,2*, A. Yu. Grishanova³, T. K. Klimenteva¹, I. V. Suhodolo¹, G. Yu. Vasukov¹, and V. V. Ivanova¹

¹Siberian State Medical University, Moskovsky Tract 2, 634050 Tomsk, Russia; fax: (3822) 53-3309; E-mail: office@ssmu.ru; milto­_bio@mail.ru

²National Research Tomsk Polytechnic University, pr. Lenina 36, 634050 Tomsk, Russia; fax: (3822) 56-3865; E-mail: tpu@tpu.ru

³Research Institute of Molecular Biology and Biophysics, Siberian Branch of the Russian Academy of Medical Sciences, ul. Timakova 2/12, 630117 Novosibirsk, Russia; fax: (383) 335-9847; E-mail: imbb@soramn.ru

^* To whom correspondence should be addressed.

Received March 3, 2014; Revision received May 12, 2014

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The influence of modified nanosized magnetite (NSM) particles (magnetic microspheres coated with chitosan and magnetoliposomes) after a single intravenous infusion of their suspensions on iron metabolism in rats has been studied. Modern physical and chemical methods (X-ray fluorescence, dynamic light scattering, transmission electron microscopy) were used for standardization of the modified NSM particles (their size, structure, ζ-potential, and concentration were determined). Atomic emission spectroscopy was used to reveal the dynamics of iron content in rat liver, spleen, lungs, and kidneys during 120 days. Colorimetric and immunoturbidimetric methods were used to determine the concentrations of plasma iron and the proteins involved in its metabolism – ceruloplasmin, transferrin, and ferritin. Their dynamics throughout the experiments were studied.

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KEY WORDS: nanomagnetite, magnetoliposomes, chitosan-modified nanomagnetite, transferrin, ceruloplasmin, ferritin

DOI: 10.1134/S0006297914110121

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