On the paper by E. R. Muslikhov, I. F. Sukhanova, and P. V. Avdonin entitled “Arachidonic Acid Activates Release of Calcium Ions from Reticulum via Ryanodine Receptor Channels in C2C12 Skeletal Myotubes” published in Biochemistry (Moscow), Vol. 79, No. 5, pp. 435-439 (2014)

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Orison O. Woolcott^1* and Md. Shahidul Islam^2,3

¹Diabetes and Obesity Research Institute, Cedars-Sinai Medical Center, 8700 Beverly Blvd, Thalians E102, Los Angeles, California, USA; fax: +1 (310) 967-3869; E-mail: Orison.Woolcott@cshs.org

²Department of Clinical Sciences and Education, Sodersjukhuset, Karolinska Institutet, Stockholm, Sweden; E-mail: shahidul.islam@ki.se

³Department of Internal Medicine, Uppsala University Hospital, Uppsala, Sweden

^* To whom correspondence should be addressed.

Received July 14, 2014

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A recent study published by Muslikhov et al. (Biochemistry (Moscow), 79, 435-439 (2014)) showed that arachidonic acid increases cytosolic Ca²⁺ concentrations in C2C12 skeletal myotubes mainly via activation of the ryanodine (RY) receptor 1. These results are consistent with the data from another study demonstrating that arachidonic acid targets RY receptor 2 in clonal and primary pancreatic β-cells (Woolcott et al., 2006). A novel and intriguing finding by Muslikhov’s group is that arachidonic acid also appears to activate the two-pore ion channel (TPC), suggesting that arachidonic acid could be a mediator in the interaction between TPCs and RY receptors.

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KEY WORDS: calcium ions, skeletal myotubes, arachidonic acid, ryanodine-sensitive channels

DOI: 10.1134/S0006297914080136

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