Role of CD11b/CD18 in Priming of Human Leukocytes by Endotoxin Glycoforms from Escherichia coli

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D. S. Kabanov^1*, S. V. Grachev^1,2, and I. R. Prokhorenko¹

¹Institute of Basic Biological Problems, Russian Academy of Sciences, 142290 Pushchino, Moscow Region, Russia; E-mail: kabanovd1@rambler.ru

²Sechenov First Moscow State Medical University, ul. Trubetskaya 8, 119992 Moscow, Russia

^* To whom correspondence should be addressed.

Received March 10, 2014; Revision received April 28, 2014

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The primary objective of this study was to determine the role of β₂ integrin α-subunit (CD11b) in the mechanism of human polymorphonuclear leukocyte (PML) priming by S or Re endotoxin glycoforms from Escherichia coli for fMLP-induced respiratory burst. Similar priming activity of S and Re endotoxin glycoforms for fMLP-induced reactive oxygen species (ROS) generation from primed PML was found. Anti-CD11b antibodies (clone ICRF 44) as well as isotype-matched immunoglobulin G1 (clone MOPC-21) do not influence the fMLP-induced ROS generation from unprimed PML. Antibodies against CD11b do not change fMLP-induced ROS generation from endotoxin-primed PML as well. The involvement of different isoforms of Fcγ receptors in fMLP-induced ROS generation from activated PML is proposed.

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KEY WORDS: lipopolysaccharide (endotoxin), β₂ integrins (CD11b/CD18), Toll-like receptor 4, Fcγ receptors, receptor cluster, immunoglobulins, anti-human CD11b antibody, reactive oxygen species

DOI: 10.1134/S0006297914080094

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