Secretion of Adipocytes and Macrophages under Conditions of Inflammation and/or Insulin Resistance and Effect of Adipocytes on Preadipocytes under These Conditions

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Yu-Tao Wei^1,2,3, Dong-Sheng Xia^3,4, Wen-Kai Yang^3,5, Xian-Guo Wang³, Xin-Zhong Chen^3*, and Nian-Guo Dong³

¹Department of Thoracic and Cardiovascular Surgery, Second Hospital Affiliated with the Medical College of Shihezi University (Department of Thoracic and Cardiovascular Surgery, Hospital of Xingjian Production and Construction Corps), Wulumuqi 830002, Xinjiang, China

²Department of Thoracic and Cardiovascular Surgery, First Hospital Affiliated with the Medical College of Shihezi University, Shihezi 832008, Xinjiang, China

³Department of Cardiovascular Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei, China; E-mail: cxz1202065@163.com; wytgyh@163.com

⁴Department of Cardiovascular Surgery, Henan Provincial People’s Hospital, Zhengzhou 450000, Henan, China

⁵Department of Cardiac Surgery, Jiangxi Provincial People’s Hospital, Nanchang 330006, Jiangxi, China

^* To whom correspondence should be addressed.

Received August 23, 2013; Revision received March 28, 2014

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The purpose of the present study was to examine changes in preadipocytes following the coculture of preadipocytes and adipocytes and the effects on the secretion of adipocytes and macrophages following induction of inflammation and insulin resistance. Mature adipocytes and RAW264.7 macrophages were treated with lipopolysaccharide and insulin to establish models of inflammation and insulin resistance, respectively. The mRNA expression levels of IL-6, MCP-1, and TNF-α in all adipocyte treatment groups were significantly greater compared with the control, and that of adiponectin was less (P < 0.05). In the RAW264.7 macrophages, the mRNA expression levels of IL-6 and TNF-α were greater than those in the control group (P < 0.05). Moreover, the results of this study confirmed that adipocytes and macrophages increased the secretion of inflammatory factors under conditions of induced inflammation and insulin resistance. In addition, 3T3-L1 adipocytes inhibited the proliferation and differentiation of preadipocytes when cocultured with adipocytes under conditions of inflammation and/or insulin resistance, and the phenotype of preadipocytes did not change.

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KEY WORDS: insulin resistance, adipocytes, preadipocytes, adipokines

DOI: 10.1134/S0006297914070086

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