Influence of the Fungus Trichoderma harzianum on the Enzyme and Polysaccharide Composition of Silene vulgaris Callus

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E. A. Günter^*, O. M. Kapustina, and Yu. S. Ovodov

Institute of Physiology, Komi Research Center, Ural Branch of the Russian Academy of Sciences, ul. Pervomaiskaya 50, 167982 Syktyvkar, Russia; fax: (8212) 241-001; E-mail: gunter@physiol.komisc.ru

^* To whom correspondence should be addressed.

Received July 10, 2012; Revision received October 29, 2012

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Activities of polygalacturonase and 1,3-β-glucanase increased in campion (Silene vulgaris) callus cells during co-cultivation with the fungus Trichoderma harzianum. This was associated with a decrease in galacturonic acid residues in the pectic polysaccharide of campion silenan and also in the production of pectin by the callus. Co-cultivation of the callus and the fungus resulted in an increase in contents of arabinose residues in the intracellular arabinogalactan and in contents of galactose residues in the extracellular arabinogalactan.

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KEY WORDS: callus, Trichoderma harzianum, Silene vulgaris, enzymes, pectic polysaccharides, silenan

DOI: 10.1134/S0006297913030115

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