Application of Recombinant Antibody Fragments for Troponin I Measurements

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E. P. Altshuler^1*, A. V. Vylegzhanina², I. A. Katrukha¹, A. V. Bereznikova¹, and D. V. Serebryanaya¹

¹Faculty of Biology, Lomonosov Moscow State University, 119991 Moscow, Russia; fax: (495) 939-1474; E-mail: eugeny.altshuler@gmail.com

²Bialexa Ltd., Simferopolsky Ave. 8, 117149 Moscow, Russia

^* To whom correspondence should be addressed.

Received August 27, 2012; Revision received September 10, 2012

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The cardiac isoform of troponin I is a reliable biomarker of damaged cardiomyocytes that accompanies such severe cardiovascular diseases as myocardial infarction. Monoclonal antibody 19C7 recognizes troponin I in the bloodstream with high affinity and specificity. Recombinant antibodies can be used to improve detection systems based on monoclonal antibodies produced with hybridoma technology. In the present study, we compare the properties of monoclonal antibody 19C7 and its recombinant fragments. It is shown that the recombinant antibody fragments demonstrate similar affinity values as monoclonal antibodies and can be applied for troponin I detection.

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KEY WORDS: monoclonal antibodies, recombinant antibodies, troponin I, affinity, fluorescence immunoassay, biotinylation

DOI: 10.1134/S0006297912120036

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