Structure of the O-Specific Polysaccharide of the Marine Bacterium Arenibacter palladensis KMM 3961^T Containing 2-Acetamido-2-deoxy-L-galacturonic Acid

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S. V. Tomshich*, V. V. Isakov, N. A. Komandrova, and L. S. Shevchenko

Pacific Institute of Bioorganic Chemistry, Far East Branch of the Russian Academy of Sciences, pr. 100-let Vladivostoku 159, 690022 Vladivostok, Russia; fax: (423) 231-4050; E-mail: tomsh@piboc.dvo.ru

^* To whom correspondence should be addressed.

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The O-specific polysaccharide was obtained by mild acid degradation of the lipopolysaccharide of the marine bacterium Arenibacter palladensis type strain KMM 3961^T and studied by chemical methods and ¹H and ¹³C NMR spectroscopy including 2D COSY, TOCSY, ¹H,¹³C HSQC, and HMBC experiments. The polysaccharide was shown to consist of tetrasaccharide repeating units containing two mannose residues (Man), one 2-acetamido-2-deoxy-D-galactose residue (D-GalNAc), and one 2-acetamido-2-deoxy-L-galacturonic acid residue (L-GalNAcA) and having the following structure:

→2)-α-D-Manp-(1→6)-α-D-Manp-(1→4)-α-L-GalpNAcA-(1→3)-β-D-GalpNAc-(1→.

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KEY WORDS: Arenibacter palladensis, marine bacteria, O-specific polysaccharide, structure, NMR spectroscopy, 2-acetamido-2-deoxy-L-galacturonic acid

DOI: 10.1134/S0006297912010105

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