Heterogeneous Structure of O-Antigenic Part of Lipopolysaccharide of Salmonella Telaviv (Serogroup O:28) Containing 3-Acetamido-3,6-dideoxy-D-glucopyranose*

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J. Kumirska^1**, H. Dziadziuszko², M. Czerwicka¹, E. A. Lubecka¹, D. Kunikowska², E. M. Siedlecka¹, and P. Stepnowski¹

¹Department of Chemistry, University of Gdańsk, Sobieskiego 18, 80-952 Gdańsk, Poland; fax: (+48-58) 523-5454; E-mail: kumirska@chem.univ.gda.pl; margo@chem.univ.gda.pl; emilial@chem.univ.gda.pl; ewas@chem.univ.gda.pl; sox@chem.univ.gda.pl

²Department of Molecular Microbiology and Serology, National Salmonella Centre, Medical University of Gdańsk, Do Studzienki 38, 80-227 Gdańsk, Poland; E-mail: halim@gumed.edu.pl; dankun@gumed.edu.pl

^* This paper is based on a presentation made at the 4th Baltic Conference on Microbial Carbohydrates, Hyytiala Forestry Field Station, Finland, September 19-22, 2010.

^** To whom correspondence should be addressed.

Received November 11, 2010; Revision received January 8, 2011

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The O-polysaccharide of Salmonella Telaviv was obtained by mild acid degradation of the lipopolysaccharide and studied by chemical methods (sugar and methylation analyses, Smith degradation, de-O-acetylation) and NMR spectroscopy. The structure of the O-polysaccharide was established. The repeating units that are proximal to the lipopolysaccharide core region mostly have a digalactose side chain and lack glucose, whereas those at the other end of the chain mostly do bear glucose but are devoid of the disaccharide side chain. This is the first structure established for the O-polysaccharide of a Salmonella serogroup O:28 (formerly M) strain characterized by subfactors O28₁ and O28₂. Knowledge of this structure and the structure of the O-polysaccharide of Salmonella Dakar (O28₁, O28₃) established earlier is crucial for determination of the exact structures associated with subfactors O28₁, O28₂, and O28₃ and elucidation of the genetic basis of the close relationship between Escherichia coli O71 and S. enterica O:28 O-antigens.

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KEY WORDS: Salmonella Telaviv, O-antigen, structural analysis, 3-acetamido-3,6-dideoxy-D-glucose

DOI: 10.1134/S000629791107008X

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