Regulation of Cyclic Electron Transport through Photosystem I in Cyanobacterium Synechocystis sp. PCC 6803 Mutants Deficient in Respiratory Dehydrogenases

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Yu. V. Bolychevtseva^1*, I. V. Elanskaya², and N. V. Karapetyan¹

¹Bach Institute of Biochemistry, Russian Academy of Sciences, Leninsky pr. 33, 119071 Moscow, Russia; fax: (495) 954-2732; E-mail: bolychev@inbi.ras.ru; nkarap@inbi.ras.ru

²Department of Genetics, Faculty of Biology, Lomonosov Moscow State University, 119992 Moscow, Russia; fax: (495) 939-2957; E-mail: ivelanskaya@mail.ru

^* To whom correspondence should be addressed.

Received November 29, 2010; Revision received January 14, 2011

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The rate of PSI mediated cyclic electron transport was studied in wild type and mutant cells of Synechocystis sp. PCC 6803 deficient in NDH-1 (M55) or succinate dehydrogenase (SDH^–) that are responsible for the dark reduction of the plastoquinone pool. Kinetics of P700 photooxidation and P700⁺ dark reduction in the presence of 5·10^–5 M 3-(3,4-dichlorophenyl)-1,1-dimethylurea have been registered as light induced absorbance changes at 810 nm resulting from illumination of cells with 730-nm actinic light for 1 sec. It is shown that in the absence of dehydrogenases the rate of dark reduction of P700⁺ in both mutants did not decrease but even increased in NDH-1-less mutant cells as compared with the rate in wild type cells. Dibromothymoquinone drastically reduced the rate of P700⁺ dark reduction both in wild type and in mutant cells. Thus, the cyclic electron transfer from ferredoxin through the plastoquinone pool to P700⁺, which is independent from dehydrogenases, takes place in all the types of cells. Preillumination of cells of wild type and both mutants for 30 min or anaerobic conditions resulted in delay of P700 photooxidation and acceleration of P700⁺ dark reduction, while the level of photosynthesis and respiration terminal acceptors (NAD(P)⁺ and oxygen) decreased. It appears that the rate of P700 photooxidation and P700⁺ dark reduction in cyclic electron transport in Synechocystis wild type and mutant cells is determined by the level of NADP⁺ and oxygen in stroma. A possible approach to evaluation of the levels of these acceptors in vivo is proposed, based on kinetic curve parameters of P700 photoconversions induced by 730-nm light with 1-sec duration.

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KEY WORDS: cyclic electron transport, NADPH, P700 redox transients, NDH-1, SDH

DOI: 10.1134/S0006297911040055

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