Major Chondroitin Sulfate Proteoglycans Identified in L6J1 Myoblast Culture

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I. I. Ermakova^1,2*, G. A. Sakuta¹, M. A. Potekhina¹, M. A. Fedorova³, R. Hoffmann³, and V. I. Morozov^1,4

¹Department of Cell Cultures, Institute of Cytology, Russian Academy of Sciences, Tikhoretsky pr. 4, 194064 St. Petersburg, Russia; fax: (812) 297-0341; E-mail: irinabiochem@mail.ru

²Pavlov Institute of Physiology, Russian Academy of Sciences, Makarova emb. 6, 199034 St. Petersburg, Russia; fax: (812) 328-0501; E-mail: tch@infran.ru

³Institute of Bioanalytical Chemistry, Center for Biotechnology and Biomedicine, Faculty of Chemistry and Mineralogy, Leipzig University, Deutscher Platz 5, 04103 Leipzig, Germany

⁴Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, pr. Toreza 44, 194223 St. Petersburg, Russia; fax: (812) 552-3012; E-mail: office@iephb.ru

^* To whom correspondence should be addressed.

Received August 2, 2010; Revision received October 19, 2010

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The major proteoglycans from L6J1 rat myoblast culture were identified. The proteoglycans were isolated from different constituents of cell culture: culture medium, extracellular matrix (ECM), and myoblasts. To identify their core proteins, the proteoglycans were treated with enzymes specifically digesting chondroitin/dermatan sulfates or chondroitin sulfates. Subsequent electrophoresis and mass spectrometry revealed versican, collagen XII, and inter-α-trypsin inhibitor classified as chondroitin sulfate proteoglycans and biglycan known to be chondroitin/dermatan sulfate proteoglycan. Versican was identified in ECM and the other proteoglycans in the culture medium. Such difference in localization is likely to be a consequence of different biological functions. Versican, collagen XII, and biglycan are synthesized by myoblasts and inter-α-trypsin inhibitor originates from fetal bovine serum (a culture medium component).

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KEY WORDS: proteoglycans, glycosaminoglycans, myoblasts

DOI: 10.1134/S0006297911030102

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