Study of Peptide Fractions from Hemolymph of Galleria mellonella

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O. S. Sribnaya^1*, P. P. Purygin¹, N. A. Klenova¹, A. K. Buryak², and E. G. Litvinova³

¹Samara State University, ul. Pavlova 1, 443011 Samara, Russia; fax: (846) 334-5417; E-mail: elektromodel@mail.ru

²Frumkin Institute of Physical Chemistry and Electrochemistry, Russian Academy of Sciences, Leninsky pr. 31/4, 119991 Moscow, Russia; fax: (495) 952-5308; E-mail: tsiv@phyche.ac.ru

³Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, ul. Institutskaya 3, 142290 Pushchino, Moscow Region, Russia; fax: (496) 733-0553; E-mail: litvinova@rambler.ru

^* To whom correspondence should be addressed.

Received January 12, 2010; Revision received March 22, 2010

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Changes in the peptide composition of hemolymph of Galleria mellonella larvae induced by their immunization have been studied, and some new peptides have been found. The composition of fractions exhibiting antibacterial activity was investigated. Known antibacterial peptides have been found in the hemolymph of control larvae and those immunized with bacteria.

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KEY WORDS: Galleria mellonella, peptides, HPLC, MALDI

DOI: 10.1134/S0006297910090117

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