Regulatory Protein Yap1 Is Involved in Response of Yeast Saccharomyces cerevisiae to Nitrosative Stress

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O. V. Lushchak¹, Y. Inoue², and V. I. Lushchak^1*

¹Department of Biochemistry, Vassyl Stefanyk Precarpathian National University, ul. Shevchenko 57, 76025 Ivano-Frankivsk, Ukraine; fax: +38(0342)714-683; E-mail: lushchak@pu.if.ua

²Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto, Japan

^* To whom correspondence should be addressed.

Received August 12, 2009; Revision received September 22, 2009

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The goal of this work was to investigate the possible involvement of protein transcription factor Yap1 in regulation of activity of antioxidant enzymes superoxide dismutase and catalase during yeast response to nitrosative stress. It was found that the inactivation of the YAP1 gene, encoding Yap1p, cancelled the activation of superoxide dismutase and catalase by NO-donors. Then, using chimeric protein Yap1–GFP, we found the accumulation of Yap1p in the nucleus in response to nitrosative stress. Therefore, we conclude that these results in combination with previous data clearly demonstrate the involvement of Yap1p in upregulation of superoxide dismutase and catalase in yeast cells in response to nitrosative stress.

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KEY WORDS: yeast, Saccharomyces cerevisiae, nitrosative stress, Yap1p, regulatory protein, fluorescence

DOI: 10.1134/S0006297910050135

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