Synthesis, Characterization, and Studies on DNA Binding of the Complex Fe(Sal₂dienNO₃·H₂O)

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Cheng-Yong Zhou^1*, Yan-Bo Wu², and Pin Yang²

¹Department of Chemistry, Changzhi University, Shanxi Changzhi 046011, PR China; fax: +86(355)220-5339; E-mail: zcy826@126.com

²Key Laboratory of Chemical Biology and Molecular Engineering of the Ministry of Education, Institute of Molecular Science, Shanxi University, Taiyuan, Shanxi 030006, PR China

^* To whom correspondence should be addressed.

Received July 8, 2009; Revision received October 15, 2009

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A new metal complex, Fe(Sal₂dienNO₃·H₂O) (where Sal is salicylaldehyde and dien is diethylenetriamine), has been synthesized and characterized. The interactions between the Fe(III) complex and calf thymus DNA has been investigated using UV and fluorescence spectra, viscosity, thermal denaturation, and molecular modeling. The cleavage reaction on plasmid DNA has been monitored by agarose gel electrophoresis. The experimental results show that the mode of binding of the complex to DNA is classical intercalation and the complex can cleave pBR322 DNA.

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KEY WORDS: DNA binding, Fe(Sal₂dienNO₃·H₂O), Fe(III) complex, calf thymus DNA

DOI: 10.1134/S0006297910040152

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