Identification and Mapping of Ten New Potential Insulators in the FXYD5-COX7A1 Region of Human Chromosome 19q13.12

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D. A. Didych, S. B. Akopov^*, E. V. Snezhkov, N. V. Skaptsova, L. G. Nikolaev, and E. D. Sverdlov

Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, 117997 Moscow, Russia; fax: (495) 330-6538; E-mail: akser@humgen.siobc.ras.ru

^* To whom correspondence should be addressed.

Received December 12, 2008; Revision received February 24, 2009

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A positive–negative selection system revealed 10 potential insulators able to block enhancer interaction with promoter in the 10⁶ bp human chromosome 19 region between genes FXYD5 and COX7A1. Relative positions of insulators and genes are in accord with the hypothesis that insulators subdivide genomic DNA into independently regulated loop domains.

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KEY WORDS: human genome, functional mapping, annotations of DNA sequences, insulator

DOI: 10.1134/S0006297909070049

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