Antibodies against DNA Hydrolyze DNA and RNA

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M. A. Krasnorutskii, V. N. Buneva, and G. A. Nevinsky^*

Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, ul. Akademika Lavrentieva 8, 630090 Novosibirsk, Russia; fax: (383) 333-1277; E-mail: nevinsky@niboch.nsc.ru

^* To whom correspondence should be addressed.

Received January 29, 2008; Revision received May 30, 2008

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In this work, rabbits were immunized with a high polymer DNA complexed with methylated BSA (mBSA) and by mBSA. It is shown that electrophoretically homogeneous preparations of polyclonal antibodies (Ab) from non-immunized rabbits and animals immunized by mBSA do not exhibit catalytic activity. Ab from the blood of rabbits immunized with the DNA-mBSA complex hydrolyzed poly(C) and different RNAs with efficiency exceeding that towards DNA by approximately 3-4 orders of magnitude. Affinity chromatography of the IgG on DNA cellulose separated the Ab into fractions hydrolyzing both RNA and DNA, and for the first time fractions that hydrolyze only RNA were found. Kinetic parameters that characterize the RNA and DNA hydrolysis by initial Ab preparations and their fractions obtained by separation on an affinity sorbent are compared.

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KEY WORDS: rabbit immunization by DNA, DNA- and RNA-hydrolyzing abzymes

DOI: 10.1134/S0006297908110114

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