Lipid Rafts Association of Synaptotagmin I on Synaptic Vesicles

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Ji-Hua Lv, Li He, and Sen-Fang Sui^*

Department of Biological Sciences and Biotechnology, State-Key Laboratory of Biomembrane and Membrane Biotechnology, Tsinghua University, Beijing 100084, China; fax: +8(610)627-93367; E-mail: suisf@mail.tsinghua.edu.cn

^* To whom correspondence should be addressed.

Received August 28, 2007; Revision received October 19, 2007

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We confirmed the raft association of synaptotagmin I (syt I) in synaptic vesicles by sucrose density gradient centrifugation, cholesterol depletion, and temperature dependence, and Ca²⁺ was found to positively regulate this association. Furthermore, using syt I mutants we found that the transmembrane domain (TMD) of syt I plays an important role in localizing syt I into the lipid rafts of synaptic vesicles, and the raft association of the TMD can be regulated by its phosphorylation status.

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KEY WORDS: lipid rafts, synaptic vesicles, synaptotagmin I

DOI: 10.1134/S0006297908030073

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