Molecular Cloning and Expression Analysis of a Novel CONSTANS-like Gene from Potato

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J. L. Guo, Q. Yang^*, F. Liang, Y. J. Xing, and Z. Wang

College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China; fax: 86-25-8439-5221; E-mail: qyang19@njau.edu.cn

^* To whom correspondence should be addressed.

Received April 23, 2007; Revision received May 28, 2007

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A full-length cDNA of a StCONSTANS-like (StCOL) gene was cloned from potato (Solanum tuberosum L.) by RT-PCR and RACE. The predicted amino acid sequence of this cDNA has a high degree of identity with other homologous members of the CO or COL family. Analysis of mRNA levels for StCOL shows that it is highly expressed in leaves and becomes weaker during tuberization; moreover, is independent of gibberellin A₃ and sucrose.

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KEY WORDS: StCONSTANS-like, cloning, expression, potato

DOI: 10.1134/S0006297907110107

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