Spectral Study of the Interaction of DNA with Benzothiazolyl-benz-alpha-chromene

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V. S. Sibirtsev^1* and A. V. Garabadzhiu²

¹Mendeleyev Russian Research Institute for Metrology, Moskovsky pr. 19, 190005 St. Petersburg, Russia; E-mail: vs1969r@mail.ru

²St. Petersburg State Technological Institute, Zagorodnyi pr. 49, 198013 St. Petersburg, Russia

^* To whom correspondence should be addressed.

Received November 20, 2006; Revision received April 19, 2007

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Absorption and luminescence excitation and emission spectra of newly synthesized 2-(4-methylphenylimino)-3-(2´-benzothiazolyl)benz-alpha-chromene (BCBT) have been studied in the presence of various DNA concentrations. BCBT is characterized by the existence of two different fluorescent systems, exhibiting radiationless fluorescence resonance energy transfer between them. In the range of molar ratios of polynucleotide/dye concentrations from 0 to 50, BCBT preferentially intercalates into DNA due to its benz-alpha-chromene fragment, whereas the 2-benzothiazolyl fragment is responsible for fluorescence.

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KEY WORDS: fluorescent DNA-specific dyes, DNA-binding ligands, spectral properties

DOI: 10.1134/S0006297907080123

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