Kinetic Models of Cyclooxygenase and Peroxidase Inactivation of Prostaglandin-H-synthase during Catalysis

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P. V. Vrzheshch^1,2*, L. A. Tsaplina^1,2, and I. S. Sakharova¹

¹School of Bioengineering and Bioinformatics, Lomonosov Moscow State University, 119992 Moscow, Russia; fax: (495) 939-4218; E-mail: peter@genebee.msu.ru; tsaplina-ludmila@yandex.ru

²International Biotechnological Center, Lomonosov Moscow State University, 119992 Moscow, Russia; fax: (495) 939-5022; E-mail: peter@genebee.msu.ru; tsaplina-ludmila@yandex.ru

^* To whom correspondence should be addressed.

Received January 15, 2007; Revision received February 14, 2007

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Kinetic models of inactivation of cyclooxygenase and peroxidase activities of prostaglandin-H-synthase (PGHS) during cyclooxygenase and peroxidase reactions catalyzed by the enzyme and also on preincubation with H₂O₂ have been developed; these models account for data obtained by the authors as well as data from the literature. Being rather simple, these models simultaneously describe the processes of cyclooxygenase and peroxidase inactivation of PGHS, using the minimal set of experimental parameters.

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KEY WORDS: prostaglandin-H-synthase, kinetic mechanism, cyclooxygenase activity, peroxidase activity, inactivation

DOI: 10.1134/S0006297907080032

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