Corrected Equations for Calculation of Constants in Enzyme Inhibition and Activation

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V. I. Krupyanko

Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, pr. Nauki 5, 142290 Pushchino, Moscow Region, Russia; fax: (495) 956-3370; E-mail: krupyanko@ibpm.pushchino.ru

Received May 24, 2006; Revision received October 30, 2006

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Equations for calculation of the constants of biparametrical types of enzyme inhibition and activation were obtained that take into account a ratio of the lengths of L vector projections representing such reactions in the three-dimensional K´_mV´I coordinate system. This allows higher accuracy of calculation and is more correct for comparison of these constants. Examples of data analysis of enzyme inhibition and activation by using the traditional equations (they do not take into account the lengths of vector projections) and corrected ones (they take into account the lengths of vector projections) are given. The corrected and traditional equations are used for calculation of the constants of biparametrical types of enzyme inhibition and activation.

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KEY WORDS: enzymes, calculation of constants of inhibition and activation

DOI: 10.1134/S0006297907040049

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