A New Method for Differential Determination of Basic Natural Forms of Indolyl-3-acetic Acid

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M. A. Gussakovskaya^* and A. N. Blintsov

Faculty of Biology, Lomonosov Moscow State University, 119992 Moscow, Russia; fax: (495) 939-4309; E-mail: magegypt@mail.ru

^* To whom correspondence should be addressed.

Received September 25, 2006; Revision received November 9, 2006

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A new ELISA method is proposed for differential quantitative determination of free (indolyl-3-acetic acid; IAA) and bound (indolyl-3-acetyl-L-aspartate) forms of natural auxins. There is similarity in results obtained by this and some traditionally used methods. The standard error of determination of the active form of IAA by our method is 1.5-2.0 times less than that using the traditional method. The method of quantitative differential determination of the main natural auxins does not require preliminary sample preparation, and this shortens assay time. The developed method has been used for practical determination of different forms of endogenous IAA in wheat and dandelion ovaries subjected to minimal treatment. This method can be used to investigate changes in the ratio of various hormonal forms of auxins that differ in their physiological activity in reproductive organs of angiosperms at various stages of reproduction.

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KEY WORDS: wheat, dandelion, ovary, endogenous auxins, ELISA, immunochemical approach, immunochemical micromethod, differential quantitative determination of IAA

DOI: 10.1134/S0006297907030133

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