Effects of Oxygen and Photosynthesis Carbon Cycle Reactions on Kinetics of P700 Redox Transients in Cyanobacterium Arthrospira platensis Cells

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Yu. V. Bolychevtseva¹, I. V. Terekhova¹, M. Roegner², and N. V. Karapetyan^1*

¹Bach Institute of Biochemistry, Russian Academy of Sciences, Leninskii pr. 33, 119071 Moscow, Russia; fax: (495) 954-2732; E-mail: nkarap@inbi.ras.ru

²Department of Plant Biochemistry, Biology Faculty, Ruhr-University-Bochum, 44780 Bochum, Germany; E-mail: matthias.roegner@ruhr-uni-bochum.de

^* To whom correspondence should be addressed.

Received July 25, 2006; Revision received September 14, 2006

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Effects of oxygen and photosynthesis and respiration inhibitors on the electron transport in photosystem I (PSI) of the cyanobacterium Arthrospira platensis cells were studied. Redox transients of P700 were induced by illumination at 730 nm and monitored as kinetics of the absorption changes at 810 nm; to block electron influx from PSII, the measurements were performed in the presence of 30 µM 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU). Inhibitors of terminal oxidases (potassium cyanide and pentachlorophenol) insignificantly influenced the fast oxidation of P700 under aerobic conditions, whereas removal of oxygen significantly decelerated the accumulation of P700⁺. In the absence of oxygen the slow oxidation of P700 observed on the first illumination was accelerated on each subsequent illumination, suggesting an activation of the carbon cycle enzymes. Under the same conditions, pentachlorophenol (an uncoupler) markedly accelerated the P700 photooxidation. Under anaerobic conditions, potassium cyanide (an inhibitor of carbon dioxide assimilation) failed to influence the kinetics of redox transients of P700, whereas iodoacetamide (an inhibitor of NADP(H)-glyceraldehyde-3-phosphate dehydrogenase) completely prevented the photooxidation of P700. Thus, the fast photooxidation of P700 in the A. platensis cells under aerobic conditions in the presence of DCMU was caused by electron transport from PSI onto oxygen, and complicated transient changes in the P700 photooxidation kinetics under anaerobic conditions (in the presence of DCMU) were due to involvement of NADP⁺ generated during the reducing phase of the carbon cycle.

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KEY WORDS: anaerobiosis, reducing phase of Calvin's cycle, inhibitors, kinetics of the P700 redox transients, NADP⁺, photosystem I

DOI: 10.1134/S0006297907030042

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