Downregulation of Genes Encoding for Subunits of Adaptor Complex-3 in Cervical Carcinomas

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A. A. Petrenko, L. S. Pavlova, A. I. Karseladze, F. L. Kisseljov, and N. P. Kisseljova^*

Institute of Carcinogenesis, N. N. Blokhin Russian Cancer Research Center, Russian Academy of Medical Sciences, Kashirskoe Shosse 24, 115478 Moscow, Russia; fax: (495) 324-1205; E-mail: natalia-kis@yandex.ru

^* To whom correspondence should be addressed.

Received May 15, 2006; Revision received June 2, 2006

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We explored the expression of four genes encoding for subunits of AP-3 in cervical tumors and cancer cell lines. Using RT-PCR we demonstrated more than twofold decrease in the levels of mRNA of AP3D1, AP3B1, AP3M1, and AP3S1 in 32, 28, 23, and 26% tumors in comparison with normal tissues of uterine cervix, respectively. The level of mRNA of at least one subunit was decreased in 28 out of 47 (60%) of tumors and in four out of five cancer cell lines in comparison to tissues adjacent to tumors. The suppression of expression of any of the subunits was revealed in 15 out of 28 cases (54%). The expression of two and more subunits was decreased simultaneously in different combinations in 13 cases (46%). This fact testifies to the lack of a common mechanism of downregulation of four subunits in tumors. There is a tendency to more frequent suppression of AP-3A expression in tumors associated with lymphatic node metastases as compared with tumors without metastases (P = 0.034). Thus, here we demonstrate for the first time the decrease in expression of genes encoding for AP-3A subunits in tumors.

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KEY WORDS: gene AP3D1, gene AP3B1, gene AP3M1, gene AP3S1, intracellular transport of proteins, uterine cervix cancer, metastases

DOI: 10.1134/S0006297906100130

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