Silybin Reduces Lipid Peroxidation of Rat Hepatocyte Membrane Caused by Cyclosporin A

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V. Moulisová^1*, M. Srbová², O. Jedlicková¹, J. Šebestian¹, and A. Jegorov³

¹Department of Plant Physiology, Faculty of Biological Sciences, University of South Bohemia, Branisovska 31, 370 05 Ceske Budejovice, Czech Republic; fax: +420-385-310-366; E-mail: vladimira.moulisova@email.cz

²Department of the Medical Chemistry and Biochemistry, The 2nd Medical Faculty, Charles University, Plzenska; 221, 150 06 Praha 5, Czech Republic; fax: +420-224-435-820; E-mail: martina.srbova@lfmotol.cuni.cz

³IVAX Pharmaceuticals s. r. o., Research Unit, Branisovska 31, 370 05 Ceske Budejovice, Czech Republic; fax: +420-385-310-331; E-mail: Alexandr_Jegorov@ivax-cz.com

^* To whom correspondence should be addressed.

Received February 26, 2006; Revision received May 17, 2006

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An effect of cyclosporin A on lipid peroxidation in isolated rat hepatocytes was tested. A significant increase in lipid peroxidation marker (the concentration of lipofuscin-like pigments) was observed in samples incubated with cyclosporin A in comparison with the control. When hepatoprotective flavonoid silybin was added, the production of lipofuscin-like pigments decreased significantly. This result indicates a potential positive role of silybin in lowering of cyclosporin A side effects associated with the production of reactive oxygen species and plasma membrane damage.

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KEY WORDS: cyclosporin A, silybin, hepatocytes, lipid peroxidation, lipofuscin-like pigments

DOI: 10.1134/S000629790610087

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