Influence of Lipopolysaccharides and Lipids A from Some Marine Bacteria on Spontaneous and Escherichia coli LPS-Induced TNF-alpha Release from Peripheral Human Blood Cells

---

E. V. Vorobeva^*, I. N. Krasikova, and T. F. Solov'eva

Pacific Institute of Bioorganic Chemistry, Far-Eastern Branch of the Russian Academy of Sciences, pr. 100-letiya Vladivostoka 159, 690022 Vladivostok, Russia; fax: (4232) 31-4050; E-mail: tikhonovaev@mail.ru

^* To whom correspondence should be addressed.

Received December 22, 2005; Revision received February 8, 2006

---

Some endotoxic properties of lipopolysaccharides (LPS) and lipids A (LA) from the marine bacteria Marinomonas communis ATCC 27118^T, Marinomonas mediterranea ATCC 700492^T, and Chryseobacterium indoltheticum CIP 103168^T were studied. The preparations tested were shown to have high 50% lethal doses (4 µg per mouse for LPS from M. mediterranea and more than 12 µg per mouse for two other LPS and LA from C. indoltheticum) and were moderate (371 ± 37 pg/ml at 10 µg/ml of C. indoltheticum LPS), weak (148 ± 5 pg/ml at 1 µg/ml of M. mediterranea LPS), and zero (LA and LPS from M. communis and LA from C. indoltheticum) inducers of tumor necrosis factor alpha (TNF-alpha) release from peripheral human blood cells. The capacity of the LA and LPS samples from marine bacteria to inhibit TNF-alpha release induced by LPS from Escherichia coli O55 : B5 (10 ng/ml) was also studied.

---

KEY WORDS: marine proteobacteria, lipopolysaccharide, lipid A, TNF-alpha

DOI: 10.1134/S000629790607008X

---