Oxidation of Guanine in Liver and Lung DNA of Prematurely Aging OXYS Rats

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E. A. Kemeleva¹, O. I. Sinitsyna¹, K. A. Conlon³, M. Berrios³, N. G. Kolosova¹, D. O. Zharkov², E. A. Vasyunina¹, and G. A. Nevinsky^1,2*

¹Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, pr. Lavrent'eva 10, 630090 Novosibirsk, Russia

²Novosibirsk Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, pr. Lavrent'eva 8, 630090 Novosibirsk, Russia; fax: (3832) 333-677; E-mail: nevinsky@niboch.nsc.ru

³Department of Pharmacological Sciences and University Microscopy Center, State University of New York at Stony Brook, NY 11794-8651, USA

^* To whom correspondence should be addressed.

Received May 4, 2005; Revision received July 4, 2005

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Immunofluorescence assay was applied for determination of 8-oxoguanine (8-oxoG) in DNA. The 8-oxoG content in liver and lung DNA of 2- and 18-month-old Wistar rats was compared with that of prematurely aging OXYS rats. It was shown that for rats of both strains, 8-oxoG content in lung DNA compared with liver DNA was 1.7-2.0-fold and 1.3-1.7-fold higher for 2- and 18-month-old rats, respectively. However, the degree of oxidative damage in liver DNA of OXYS rats was 2.4- (p < 0.01) and 1.5-fold (p < 0.05) higher for 2- and 18-month-old animals, respectively, than that in liver DNA of Wistar rats. Oxidation of guanine in lung DNA of OXYS rats was 2- (p < 0.01) and 1.7-fold (p < 0.05) higher for 2- and 18-month-old animals, respectively, than that in lung DNA of Wistar rats. The data indicate that elevated DNA oxidative damage in various organs of OXYS rats may be an important factor of accelerated aging and progression of age-related diseases--cataract, macular dystrophy, hypertension, osteoporosis, cognitive and behavioral dysfunctions, and also lung and liver pathologies.

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KEY WORDS: oxidative stress, 8-oxoguanine, premature aging, OXYS rats

DOI: 10.1134/S0006297906060046

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