Programmed Cell Death in Plants: Effect of Protein Synthesis Inhibitors and Structural Changes in Pea Guard Cells

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E. V. Dzyubinskaya¹, D. B. Kiselevsky¹, L. E. Bakeeva², and V. D. Samuilov^1*

¹Department of Physiology of Microorganisms, Biological Faculty, Lomonosov Moscow State University, 119992 Moscow, Russia; fax: (495) 939-3807; E-mail: vdsamuilov@mail.ru

²Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 119992 Moscow, Russia; fax: (495) 939-3181

^* To whom correspondence should be addressed.

Received May 11, 2005; Revision received November 16, 2005

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Pea leaf epidermis incubated with cyanide displayed ultrastructural changes in guard cells that are typical of apoptosis. Cycloheximide, an inhibitor of cytoplasmic protein synthesis, and lincomycin, an inhibitor of protein synthesis in chloroplasts and mitochondria, produced different effects on the dynamics of programmed death of guard cells. According to light microscopy data, cycloheximide reinforced and lincomycin suppressed the CN^--induced destruction of cell nuclei. Lincomycin lowered the effect of cycloheximide in the light and prevented it in the dark. According to electron microscopy data, the most pronounced effects of cycloheximide in the presence of cyanide were autophagy and a lack of apoptotic condensation of nuclear chromatin, the prevention of chloroplast envelope rupturing and its invagination inside the stroma, and the appearance of particular compartments with granular inclusions in mitochondria. Lincomycin inhibited the CN^--induced ultrastructural changes in guard cell nuclei. The data show that programmed death of guard cells may have a combined scenario involving both apoptosis and autophagy and may depend on the action of both cytoplasm synthesized and chloroplast and mitochondrion synthesized proteins.

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KEY WORDS: programmed cell death, apoptosis, autophagy, cycloheximide, lincomycin, guard cells, nucleus, chloroplasts, mitochondria, light microscopy, electron microscopy, Pisum sativum L.

DOI: 10.1134/S0006297906040079

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