Tissue Specificity of Methylation of Cytosines in Regulatory Regions of Four Genes Located in the Locus FXYD5-COX7A1 of Human Chromosome 19: Correlation with Their Expression Level

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T. V. Chalaya, S. B. Akopov^*, L. G. Nikolaev, and E. D. Sverdlov

Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, 117997 Moscow, Russia; fax: (7-495) 330-6538; E-mail: akser@humgen.siobc.ras.ru

^* To whom correspondence should be addressed.

Received October 17, 2005; Revision received November 3, 2005

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In this study, we compared degree of methylation of selected CpG sites in CCGG sequences located in promoter regions of four human genes with expression level of these genes in several human cell lines and tissues. These genes were subdivided into two groups according to the dependence of their expression on CpG methylation in the 5´-regions. The first group, characterized by clear correlation of methylation with the transcription level, includes housekeeping gene COX6B (the absence of methylation unambiguously correlates with expression) and urothelium-specific uroplakin gene (the methylation coincides with absence of expression). The second group includes genes that are expressed in many, but not all tissues and cells. For these genes (LEAP-1 and ATP4A), there was no correlation between methylation and expression. It is possible that methylation provides some basal level of gene repression, which is overcome by binding of tissue-specific transcription factors, whereas lack of methylation gives the opportunity for gene expression in various cells and tissues.

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KEY WORDS: human genome, cytosine methylation, gene regulation

DOI: 10.1134/S0006297906030096

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