Cloning and Characterization of Two EREBP Transcription Factors from Cotton (Gossypium hirsutum L.)

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Hongying Duan¹, Fuguang Li², Xudong Wu³, Dongmei Ma³, Mao Wang¹, and Yuxia Hou^3*

¹College of Biological Sciences, China Agriculture University, Beijing 100094, P. R. China

²Cotton Research Institute, Chinese Academy of Agricultural Sciences, Henan Anyang 455004, P. R. China

³College of Science, China Agriculture University, Beijing 100094, P. R. China; fax: 86-10-627-33824; E-mail: houyuxia@tsinghua.org.cn

^* To whom correspondence should be addressed.

Received September 5, 2005; Revision received October 10, 2005

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In this research, two EREBP (ethylene response element binding protein) genes were isolated by the yeast one-hybrid system, named GhEREB2 and GhEREB3, and both have one intron in their coding regions. The deduced amino acid sequences of GhEREB2 and GhEREB3 have some typical features of transcription factors, one potential basic nuclear-localization signal, one possible acidic activation domain, and one conserved DNA binding domain, and they show high similarity, especially in the DNA-binding domain. GhEREB2 was expressed in roots, stems, and leaves (low levels), but GhEREB3 was only expressed in leaves. Furthermore, the expression of GhEREB2 and GhEREB3 was induced by ethylene and jasmonic acid. In addition, GhEREB2 and GhEREB3 proteins specifically bind to a GCC-box and strongly activate the expression of HIS3 and LacZ reporter genes in yeast. Collectively, these results suggest that GhEREB2 and GhEREB3 might be involved as positive transcription factors in biotic stress signal transduction pathways.

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KEY WORDS: AP2/EREBP domain, EREBP protein, GCC-box, GhEREB2, GhEREB3, cotton

DOI: 10.1134/S0006297906030084

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