Single Step Immobilized Metal Ion Affinity Precipitation/Chromatography Based Procedures for Purification of Concanavalin A and Cajanus cajan Mannose-Specific Lectin

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A. Naeem, R. H. Khan^*, and M. Saleemuddin

Interdisciplinary Biotechnology Unit and the Department of Biochemistry, Aligarh Muslim University, Aligarh-202002, India; fax: 91-571-272-1776; E-mail: rizwanhkhan@hotmail.com; rizwanhkhan1@yahoo.com

^* To whom correspondence should be addressed.

Received March 1, 2005; Revision received May 25, 2005

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Concanavalin A and a mannose-specific lectin could be precipitated specifically from extracts of jack bean and Cajanus cajan seeds, respectively, using metal charged EGTA. Single step purification of the lectins was also possible using iminodiacetic acid-Sepharose charged with metal ions. Nondenaturing electrophoresis in polyacrylamide gel and that performed in presence of SDS ascertained homogeneity of the isolated lectins. The migration behavior of the purified lectins was comparable with those of the lectins purified using alternative procedures.

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KEY WORDS: affinity chromatography, Cajanus cajan, concanavalin A, metal affinity precipitation, polyhistidine tails, single step purification

DOI: 10.1134/S0006297906010081

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