Spectral Study of Interactions of 4,8,4´-Trimethylpsoralen and 4,4´-Dimethylangelicin Dyes with DNA

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V. S. Sibirtsev^1*, A. Yu. Tolmachev², M. V. Kovaleva³, A. V. Garabadzhiu³, and V. F. Traven²

¹Mendeleyev Russian Research Institute for Metrology, Moskovsky pr. 19, 190005 St. Petersburg, Russia; fax: (7-812) 327-9776; E-mail: vs1969r@mail.ru

²Mendeleyev University of Chemical Technology of Russia, Miusskaya pl. 9, 125047 Moscow, Russia

³St. Petersburg State Technological Institute, Zagorodnyi pr. 49, 190013 St. Petersburg, Russia

^* To whom correspondence should be addressed.

Received March 26, 2004; Revision received June 24, 2004

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Absorption and fluorescence spectra for six new synthetic dyes of 4,8,4´-trimethylpsoralen and 4,4´-dimethylangelicin derivatives containing various terminal substituents at 5´-position have been investigated in different environments using a wide range of the DNA/ligand concentrations. Various spectral and binding characteristics of the DNA-ligand systems have been determined. General principles characterizing mechanisms responsible for changes in the fluorescent properties of nucleotide-specific dyes have been proposed; they take into consideration chemical structure of the dyes, properties of the environment, and degree of sorption on substrate.

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KEY WORDS: DNA, fluorescence dyes, spectral and binding properties

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