Influence of Seizures on Lipids of Homogenate and Neuronal and Glial Nuclei of Rat Neocortex

T. P. Kulagina^1*, N. A. Shevchenko², and V. I. Arkhipov²

¹Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino 142290, Moscow Region, Russia; fax: (7-0967) 33-0509; E-mail: kulagina@icb.psn.ru

²Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino 142290, Moscow Region, Russia; fax: (7-0967) 33-0553; E-mail: arkhipov@iteb.ru

^* To whom correspondence should be addressed.

Received March 9, 2004; Revision received May 7, 2004

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Lipid composition of homogenate and neuronal and glial nuclei of the brain cortex of Wistar rats was studied under normal conditions and after seizures induced by injection of picrotoxin. Seizures increased contents of lysophosphatidylcholine, sphingomyelin, and total phospholipids in the homogenate. In neuronal nuclei contents of total phospholipids, sphingomyelin, phosphatidylcholine, and phosphatidylserine decreased, and contents of free fatty acids and lysophosphatidylcholine increased. In glial nuclei content of total phospholipids decreased and content of free fatty acids increased. The role of changes in the lipid composition of the neocortex cells during seizures and the involvement of lipid messengers in signal mechanisms are discussed.

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KEY WORDS: picrotoxin, rat brain cortex, neuronal nuclei, glial nuclei, lipids

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