Isolation, Purification, and Crystallization of Aspartate Aminotransferase from Wheat Grain

V. M. Kochkina

Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, ul. Vavilova 32, Moscow 119991, Russia; fax: (7-095) 135-1405; E-mail: belj@eimb.ru

Received November 27, 2003; Revision received April 1, 2004

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A procedure for isolation and purification of aspartate aminotransferase from wheat grain includes chromatography on DEAE cellulose, acidification-alkalization, precipitation with protamine sulfate, fractionation with ammonium sulfate, and chromatography on hydroxyapatite. The yield of protein was 27% with 95% purity. Crystals of the enzyme (0.05 × 0.025 × 0.015 mm³) were obtained from ammonium sulfate solution.

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KEY WORDS: wheat grain, aspartate aminotransferase, purification, crystallization

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