Effect of Polysaccharides and Human Plasma Lipoproteins on the Secretion of Cystatin C by Peritoneal Macrophages from Normal and Tumor Bearing Mice

O. N. Poteryaeva^1*, L. M. Polyakov¹, T. A. Korolenko², T. V. Zueva¹, G. S. Russkikh¹, and L. E. Panin¹

¹Institute of Biochemistry and ²Institute of Physiology, Siberian Branch of the Russian Academy of Medical Sciences, ul. Akademika Timakova 2, Novosibirsk 630117, Russia; fax: (383-2) 35-6811; E-mail: ibch@soramn.ru

^* To whom correspondence should be addressed.

Received February 17, 2003; Revision received April 1, 2003

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Intact peritoneal macrophages in vitro secreted the cysteine proteinase inhibitor cystatin C. Polysaccharides stimulated cystatin C secretion: lipopolysaccharide < carboxymethylated beta-D-glucan < sulfoethylated beta-D-glucan. Human plasma low-density- (LDL) and high-density lipoproteins (HDL) are still more potent inducers of cystatin C secretion by macrophages. Peritoneal macrophages from mice with experimental HA-1 hepatoma compared to those from intact mice secreted more cystatin C with maximum polysaccharide-stimulated secretion after 30 min of incubation. LDL and HDL induced cystatin C secretion by tumor macrophages also.

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KEY WORDS: cystatin C, peritoneal macrophage, lipoprotein, hepatoma HA-1

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