Purification and Some Properties of Lysostaphin, a Glycylglycine Endopeptidase from the Culture Liquid of Staphylococcus simulans biovar staphylolyticus

T. V. Fedorov¹, V. I. Surovtsev^1*, V. Z. Pletnev², M. A. Borozdina¹, and V. V. Gusev¹

¹State Research Center for Applied Microbiology, Ministry of Health of Russian Federation, Obolensk, Moscow Region, 142279 Russia. fax. (0967) 36-0010; E-mail. center@gncpm.serpukhov.su

²Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, Moscow, 117997 Russia; fax: (095) 335-7103; E-mail: root@mail.ibch.ru

^* To whom correspondence should be addressed.

Received October 2, 2001

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This work presents a method for purification of lysostaphin, a glycylglycine endopeptidase, from the culture liquid of S. simulans biovar staphylolyticus to homogeneity in a few steps. The method includes ultrafiltration and ion-exchange and hydrophobic chromatographies. The enzyme was isolated in preparative amounts with the yield of 51%. Some physical and chemical properties of the enzyme are described.

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KEY WORDS: lysostaphin, Staphylococcus simulans biovar staphylolyticus, glycylglycine endopeptidase, purification, properties, hydrophobic chromatography

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