Structure of O-Specific Polysaccharide from Pseudoalteromonas nigrifaciens Strain KMM 161

R. P. Gorshkova^*, E. L. Nazarenko, V. A. Zubkov, E. P. Ivanova, N. M. Gorshkova, and V. V. Isakov

Pacific Institute of Bioorganic Chemistry, Far East Branch of the Russian Academy of Sciences, pr. 100-letiya Vladivostoka 159, Vladivostok, 690022 Russia; fax: (4232) 31-4050; E-mail: elnaz@piboc.dvo.ru

^* To whom correspondence should be addressed.

Received April 16, 2001; Revision received July 10, 2001

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On mild acid degradation of the lipopolysaccharide of the marine microorganism Pseudoalteromonas nigrifaciens KMM 161 an O-specific polysaccharide containing D-galactose, 2-acetamido-2-deoxy-D-glucose, 3,6-dideoxy-3-(4-hydroxybutyramido)-D-galactose, and 2-acetamido-2-deoxy-L-guluronic acid residues was obtained. From the results of Smith degradation, O-deacetylation of the polysaccharide, and NMR spectroscopy the following structure of the tetrasaccharide repeating unit of the O-specific polysaccharide was established [figure]. It should be noted that the same structure occurs in the antigenic polysaccharide of Pseudoalteromonas nigrifaciens KMM 158 described earlier as Alteromonas macleodii 2MM6.

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KEY WORDS: Pseudoalteromonas nigrifaciens, O-specific polysaccharide, 4-hydroxybutanoic acid,NMR spectroscopy

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