O-Specific Polysaccharide of the Marine Bacterium “Alteromonas marinoglutinosa” NCIMB 1770

N. A. Komandrova^*, S. V. Tomshich, V. V. Isakov, and L. A. Romanenko

Pacific Institute of Bioorganic Chemistry, Far East Division of the Russian Academy of Sciences, pr. 100-letiya Vladivostoka 159, Vladivostok, 690022 Russia; fax: 7 (4232) 314-050; E-mail: piboc@stl.ru

^* To whom correspondence should be addressed.

Received December 25, 2000; Revision received February 12, 2001

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The O-specific polysaccharide of the marine bacterium “Alteromonas marinoglutinosa” NCIMB 1770 was obtained by mild acid degradation of the corresponding lipopolysaccharide and found to contain D-galactose, N-acetyl-D-glucosamine, and N-acetyl-D-mannosamine residues in equimolar ratio. Based on methylation analysis, periodate oxidation, and ¹³C-NMR spectroscopy data of native and modified polysaccharides, the following structure of the trisaccharide repeating unit of the O-specific polysaccharide was established:

                    beta-D-ManpNAc
                            1
                            |
                            V
                            4
-->3)-alpha-D-Galp-(1-->3)-beta-D-GlcpNAc-(1-->

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KEY WORDS: “Alteromonas marinoglutinosa”, marine bacteria, lipopolysaccharide, O-specific polysaccharide, structure, NMR spectroscopy

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