Influence of cis-[Re₂GABA₂Cl₄]Cl₂ on the Antioxidant Defense System Parameters of Normal Human Blood

I. V. Pirozhkova-Patalah^* and N. I. Shtemenko

Department of Biophysics and Biochemistry, Dniepropetrovsk National University, Nauchnyi Pereulok 13, Dniepropetrovsk, 49050 Ukraine; fax: +38-0562-46-55-23; E-mail: ppirina@aport.ru

^* To whom correspondence should be addressed.

Received April 20, 2000; Revision received January 31, 2001

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The effect of the rhenium complex cis-[Re₂GABA₂Cl₄]Cl₂ on the antioxidant parameters of normal human blood in vitro have been studied. The results suggest that the complex influences various enzymes in the cascade of reactions utilizing active oxygen metabolites. However, the manifestation of this activity varies over the studied concentration range of the complex in the preincubation medium (10^-12-10^-4 M), so the effects appear to be concentration-dependent. The largest differences in antioxidant parameters in comparison with control were observed for the concentrations 10^-8, 10^-5, and 10^-4 M. Thus, correlations between the peroxidation level, superoxide dismutase (SOD) activity, antioxidant factor (F), and indexes of resistance of erythrocytes for hemolysis (TR) were found.

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KEY WORDS: cis-[Re₂GABA₂Cl₄]Cl₂ complex, antioxidant parameters, human blood

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