Natural Hidden Autoantibodies React with Negatively Charged Carbohydrates and Xenoantigen Bdi

I. V. Lekakh^1*, N. V. Bovin², G. P. Bezyaeva¹, and A. M. Poverenny¹

¹Medical Radiology Research Center, Russian Academy of Medical Sciences, ul. Koroleva 4, Obninsk, 249020 Russia; fax: (095) 956-1440; E-mail: MRRC@OBNINSK.RU

²Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, Moscow, 117871 Russia; fax: (095) 330-5592; E-mail: bovin@carb.siobc.ras.ru

^* To whom correspondence should be addressed.

Received November 1, 1999; Revision received September 2, 2000

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Immunoglobulin preparations from sera of healthy donors contain polyspecific autoantibodies interacting with DNA and other charged antigens. These antibodies belong to the IgG class and can exist in the free or hidden state. The hidden antibody activity can be revealed after ion-exchange chromatography on QAE-Sephadex A-50. Immunoenzyme assay was used to assess the interactions of both free and hidden antibodies with different carbohydrates. The hidden antibodies were only able to interact with different polyanionic carbohydrates and neutral xenoantigen Bdi.

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KEY WORDS: natural antibodies, hidden antibodies, polyspecificity of antibodies, antibodies against DNA, antibodies against carbohydrates

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