Receptor-Mediated Transfer of DNA-Galactosylated Poly-L-lysine Complexes into Mammalian Cells in vitro and in vivo

E. B. Dizhe, B. N. Akifiev, B. V. Missul, S. V. Orlov, O. V. Kidgotko, V. E. Sukonina, A. D. Denisenko, and A. P. Perevozchikov^*

Institute of Experimental Medicine, Russian Academy of Medical Sciences, ul. Akademika Pavlova 12, St. Petersburg, 197376 Russia; fax: (812) 234-9489; E-mail: app@usr1.iem.ras.spb.ru

^* To whom correspondence should be addressed.

Received February 15, 2000; Revision received July 6, 2000

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With the goal of developing non-viral techniques for exogenous gene delivery into mammalian cells, we have studied receptor-mediated gene transfer using complexes of plasmid DNA and galactosylated poly-L-lysine, poly(L-Lys)Gal. To evaluate the optimal parameters for efficient gene transfer into human hepatoma HepG2 cells by the DNA-poly(L-Lys)Gal complexes, the bacterial reporter genes lacZ and cat were used. Examination of the reporter gene expression level showed that the efficiency of DNA delivery into the cells depends on the structure of DNA-poly(L-Lys)Gal complexes formed at various ionic strength values. The efficiency of DNA transfer into the cells also depends on DNA/poly(L-Lys)Gal molar ratio in the complexes. Plasmid vector carrying human apolipoprotein A-I (apoA-I) gene was injected as its complex with poly(L-Lys)Gal into rat tail vein. Some level of ApoA-I was detected in the serum of the injected rats. Also, the human apoA-I-containing plasmid was found to be captured specifically by the rat liver cells and transported into the cell nuclei, where it can persist as an episome-like structure for at least a week. After repeated injections of DNA-poly(L-Lys)Gal complexes, the level of human ApoA-I in rat serum increases, probably, due to accumulation of functional human apoA-I gene in the liver cell nuclei. The data seem to be useful for the development of non-viral approaches to gene therapy of cardiovascular diseases.

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KEY WORDS: gene delivery, mammalian cells, bacterial reporter genes, lacZ gene, cat gene, human apolipoprotein A-I gene, DNA complex, galactosylated poly-L-lysine, persistence, exogenous DNA, episome-like structure, cell nucleus

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