Structure of Anionic Carbohydrate-Containing Cell Wall Polymers in Several Representatives of the Order Actinomycetales

Yu. I. Kozlova¹, G. M. Streshinskaya¹, A. S. Shashkov², L. I. Evtushenko³, E. Yu. Gavrish³, and I. B. Naumova^1*

¹School of Biology, Lomonosov Moscow State University, Moscow, 119899 Russia; E-mail: naumova@microbiol.bio.msu.ru or i_naumova@mail.ru

²Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky pr. 47, Moscow, 117913 Russia; fax: (095) 135-5328

³Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Moscow Region, 142292 Russia; fax: (095) 923-3602

^* To whom correspondence should be addressed.

Received March 1, 2000

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A new teichoic acid was identified in the cell walls of Streptomyces griseoviridis VKM Ac-622^T, Streptomyces sp. VKM Ac-2091, and Actinoplanes campanulata VKM Ac-1319^T. The polymer is poly(glycosylglycerol phosphate). The repeating units of the polymer, alpha-galactopyranosyl-(1-->3)-2-acetamido-2-deoxy-beta-galactopyranosyl-(1-->1)-glycerols, are in phosphodiester linkage at C-3 of glycerol and C-6 of galactose. The structures of cell wall teichoic acids in the strains Streptomyces chryseus VKM Ac-200^T and “Streptomyces subflavus” VKM Ac-484 similar in morphology and growth characteristics are also identical: 1,5-poly(ribitol phosphate) substituted at C-4(2) by 2-acetamido-2-deoxy-beta-glucopyranosyl residues and 1,3-poly(glycerol phosphate). The taxonomic aspects of these results are discussed.

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KEY WORDS: Streptomyces, Actinoplanes, cell wall, poly(glycosylglycerol phosphate), poly(polyol phosphates), taxonomy, ¹³C-NMR spectroscopy

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